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An insulating cone is placed over the end from which the collection tube protrudes to avoid subjecting the semen to temperature shock. Therefore, the use of sperm from this fraction can have a positive effect on fertilization and embryonic development. Some programs also recommended cleansing the penis followed with rinsing and drying to remove any soap or water. Although there are no definitive studies to this regard, the pre-ejaculatory phase or pre-seminal fluid does not contain sperm; it is a colourless secretion from the Cowper's glands which is expelled to lower the acidity of the urethra. Often the semen sample must be produced in the fertility clinic , in a room set aside for this purpose. Social conditions may influence sperm quality.
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