Sperm vitrification and fixation

As first step in cryopreservation experiments, the mammalian cells were trypsinized, washed, and taken up in suspension in the indicated media. To remove potential contamination on the tubes outer side, they may be washed and sterilized after thawing. Viability of mammalian cells in SPRF tubes. Chetverikova, E. The main advantages of SPRF are especially interesting for large scale bio-banking, where storage space becomes an important issue, and for storage of clinical or biosafety material, where contamination of or from the sample has to be strictly avoided. However, slow freezing showed a more significant difference in relation to the control Therefore, cell morphology, oxidative stress, DNA damage,mitochondrial activity and structure were measure after slow freezing and vitrification.

So far, the research looks promising when comparing slow-freeze to vitrification. Reactive oxygen species and human spermatozoa: physiology and pathology. IVF protocol using sperm from epididymides following refrigerated transport Sep